報告書番号

U97081

タイトル（和文）

商用周波磁界と免疫系（その１）-1.0 µTrms水平、および、1.4 µTrms回転磁界に曝露した抗CD3抗体刺激マウスのサイトカイン産生-

タイトル（英文）

Power frequency magnetic field and immune system I. - Cytokine production of anti-CD3 antibody treated mice exposed to 1.0 µTrms horizontal or 1.4 µTrms circularly polarized magnetic field -

概要 （図表や脚注は「報告書全文」に掲載しております）

免疫系への磁界影響を明らかにすることは，磁界による腫瘍促進・阻害のメカニズムを解明する上で重要である。研究目的は，免疫情報伝達物質（サイトカイン）の産生能が磁界で変化するか否かを解明することである。曝露したマウス体内のＴリンパ球を抗体で刺激し，血清と脾臓の３種類のサイトカインを定量した。無曝露状態や曝露期間、系統差を検討したのち，１群１２匹のマウスに１．０ｍｉｃｒｏＴの水平，または１．４ｍｉｃｒｏＴ回転磁界を６週間曝露した。その結果，水平磁界ではサイトカイン産生の変化は少なく，回転磁界で変化が見られ統計学的に有意な場合もあった。傾向としては，ＩＮＦ－ａｌｐｈａが増加，ＩＬ－６が減少であった。しかし，変動の見られたサイトカインの種類や増減が同一条件下での繰り返し実験で一貫しておらず，明確な磁界影響ではなかった。今後は，より強い磁界で同様の曝露実験を実施する。

概要 （英文）

The aim of this research is to examine possible effects of MF exposures on production of endogenous cytokines that play crucial roles in intercellular communication in the immunologic system. Mice were housed in identical exposure facilities, and they were exposed to MFs under various conditions. At the termination of exposure, T-cells in the exposed and sham-exposed animals were stimulated with an anti-CD3 epsilon antibody treatment. The animals were sacrificed at certain time points after the treatment. Produced three cytokines, tumor necrosis factor-alpha (TNF-α), interleukin-6 (IL-6), and interferon-gamma (IFN-γ), in sera and spleens were measured with enzyme-linked immunosorbent assays. Statistical evaluations were always performed between MF-exposed animals and corresponding sham-exposed animals. We first determined the bases of these cytokine productions in the two groups of mice without an MF exposure. No differences between those two groups of animals in either cytokine were evident. Optimal exposure term, 1 to 9 weeks, and strain differences, ddY and C57BL/6, were also explored. Thereafter, groups of twelve ddY mice were exposed to 50 Hz, 1.0 µTrms horizontal MF or 1.4 µTrms circularly polarized MF for 6 weeks. Control animals were treated as the same except MF exposures. The results indicated that there appear to be less change by the horizontal MF exposure whereas several changes, some were statistically significant, were detected by circularly polarized MF exposures. Overall tendency included increases in TNF-α, decreases in IL-6, and no alteration in IFN-γ. The reproducibility of the results, however, was not preserved among replications, namely both increase and decrease in each cytokine were detected in repeated experiments under the same conditions. This leads the authors to conclude that obtained data did not support the definitive effect of MF exposure on the endogenous cytokine production under conditions examined. Additional experiments with much higher intensity of MF exposure are underway to investigate the possible MF influences on the production of endogenous cytokines in the exposed mice that are stimulated with an anti-CD3 antibody treatment.

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