報告書番号

U96021

タイトル（和文）

挿し木と組織培養における合成オーキシン類の施用効果

タイトル（英文）

EFFECT OF APPLIED SYNTHETIC AUXIN ON ROOT GROWTH IN PLANTLET PROPAGATION BY CUTTAGE AND TISSUE CULTURE

概要 （図表や脚注は「報告書全文」に掲載しております）

挿し木繁殖における４－Ｃ１－ＩＡＡとＴＦＩＢＡおよび組織培養におけるＴＦＩＢＡの施用効果を調べた。ニチニチソウ，ブドウ，サツキのいずれにおいても，４－Ｃ１－ＩＡＡの施用により，発根作用および根の成長に対して促進効果が認められた。根の成長促進効果が最も大きく現れる４－Ｃ１－ＩＡＡの施用濃度は植物種の違いにより大きく異なった。また，ＴＦＩＢＡはイチゴの生長点培養でシュートの形成を促進させる，発根を促進させる，カルス化を抑制するなどの作用を持つことが明らかになった。したがって，４－Ｃ１－ＩＡＡとＴＦＩＢＡについては苗生産分野等での発根促進剤としての利用が可能であると考えられた。

概要 （英文）

WE INVESTIGATED THE EFFECTS OF 4-CHLOROINDOLE-3-ACETIC ACIT(4-C1-IAA)AND 4,4,4-TRIFLUORO-3-(INDOLE-3-)BUTYRIC ACID(TFIBA)ON ROOT GROWTH IN PLANTLET PROPAGATION BY CUTTAGE AND TISSUE CULTURE. 1.EFFECTS OF 4-C1-IAA AND TFIBA ON ROOT GROWTH IN CUTTAGE (1)4-CI-IAA TREATMENT INCREASED THE ROOT ELONGATION,NUMBER OF ROOTS AND ROOT WEIGHT OF PERIWINKLE.THE MOST EFFECTIVE CONCENTRATION OF 4-C1-IAA TO PROMOTE ROOT GROWTH WAS 20 PPM.(2)THE ROOTING ABILITY OF GRAPE WAS STIMULATED BY EITHER 4-C1-IAA AT 20 PPM OR IBA AT 250 PPM.THE ROOTING RATES FOR4-C1-IAA AND IBA TREATMENT WERE ABOUT 80%,OR 2.2 TIMES HIGHER THAN WITH NO TREATMENT (0 PPM).MOREOVER,4-C1-IAA AND IBA TREATMENT INCREASED ROOT ELONGATION,NUMBER OF ROOTS AND ROOT WEIGHT.(3)4-C1-IAA AND TFIBA ALSO PROMOTED ROOT GROWTH IN AZALEA.THE MOST EFFECTIVE CONCENTRATION OF 4-C1-IAA AND TFIBA WAS 2000 PPM AND 4000 PPM,RESPECTIVELY.THE ROOT GROWTHPROMOTION OF 4-C1-IAA WAS THE SAME AS THAT OF IBA,BUT THAT OF TFIBA WAS HIGHER THAN THAT OF IBA. 2.EFFECTS OF TFIBA ON ROOT GROWTH OF TISSUE-CULTURED STRAWBERRY TFIBA AND 6-BENZYL AMINOPURIN(BA)WERE USED IN IN-VITRO BIOASSAY SYSTEMS FOR THE APICAL MERISTEM.EXPLANTS EXCISED FROM RUNNERS WERE CULTURED ON MODIFIED MURASHIGE-SKOOG MEDIUM CONTAINING TFIBA AND BA AT CONCENTRATIONS BETWEEN 0 TO 1 PPM. (1)TFIBA PROMOTED ROOTING AND ACCELERATED GROWTH OF THE APICAL BUG,BUT DID NOT INDUCE CALLUS FORMATION.(2)THE MOST EFFECTIVE CONCENTRATION OF TFIBA FOR ROOTTING WAS 0.5 PPM.(3)USING TFIBA WITH BA INDUCED MULTIPLE SHOOTS,BUT ROOTTING WAS INHIBITED. 4-CI-IAA AND TFIBA CAULD BOTH BE USED AS ROOTING CHEMICALS IN PLANTS PRODUCTION.

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