報告書番号

U95038

タイトル（和文）

排水中の窒素除去に関する研究２．チューブ状ゲル内部の固定化微生物の挙動

タイトル（英文）

DEVELOPMENT OF BIOLOGICAL WASTEWATER TREATMENT SYSTEM FOR NITROGEN REMOVAL 2.BEHAVIOR OF IMMOBILIZED AMMONIA-OXIDIZING BACTERIA AND DENITRIFYING BACTERIA IN TUBULAR GEL OF BIOREACTOR

概要 （図表や脚注は「報告書全文」に掲載しております）

アンモニア酸化菌Ｎｉｔｒｏｓｏｍｏｎａｓｅｕｒｏｐａｅａと脱窒菌Ｐａｒａｃｏｃｃｕｓｄｅｎｉｔｒｉｆｉｃａｎｓを包埋したチューブ状ゲルにより，アンモニアを含んだ模擬排水の連続処理を行い，ゲルのアンモニア除去能力とゲル内部の微生物の挙動について検討した。その結果，アンモニア除去能力は３３日間安定しており，連続処理方式によってもチューブ状ゲルを用いたリアクターの運用が可能であることが分かった。また，ゲル内部における両菌株の増殖領域から，ゲル内部は好気な微生物反応が起こるゲル表面から０．１ｍｍまでに領域，好気と■気の微生物反応が共存する０．１から０．２ｍｍまでの領域，■気な微生物反応が起こる０．２ｍｍよりも奥の領域の３つに分けられることが示唆された。従って，リアクターの容量当たりの能力をさらに向上させられるために，チューブ状ゲルを細くし表面積を大きくした形状にすることが可能と考えられる。

概要 （英文）

OUR BIOREACTOR FOR NITROGEN REMOVAL CONSISTSOF TUBULAR POLYMERIC GELS CONTAINING THE AMMONIA-OXIDIZING BACTERIA,NITROSOMONAS EUROPAEA AND THE DENITRIFIER,PARACOCCUS DENITRFICANS. THE OUTER SURFACE OF THE TUBULAR GEL AEROBICALLY CONTACTS WASTEWATER CONTAINING AMMONIA. N.EUROPAEA IN THE GEL OXIDIZES AMMONIA TO NITRITE AEROBICALLY. ON THE OTHER HAND,THE INSIDE OF THE GEL ANAEROBICALLY CONTACTS ETHANOL SOLUTION AS AN ELECTRON DONOR FOR DENITRIFICATION. P.DENITRIFICANS IN THE GEL REDUCES THE NITRITE TO NITROGEN GAS ANAEROBICALLY. THEREFORE,AMMONIA IN THE WASTEWATER IS EFFECTIVELY REMOVED WITHOUT GENERATING NITRATE,NITRATE,AND NITROUS OXIDE. ARTIFICIAL WASTEWATER CONTAINING 100 MG-N/1 OF AMMONIA WAS TREATED CONTINUOUSLY BY A SINGLE-PASS REACTOR CONTAINING THE TUBULAR GEL.CHANGES IN THE GEL ACTIVITY AND BACTERIAL DISTRIBUTIONS IN THE GEL WERE MONITORED FOR 33 DAYS.THE AMMONIA REMAIAL RATE OF THE SINGLS-PASS REACTOR WAS ALMOST CONSTANT AT 0.2G-N/H FOR 1SQUARE M OF GEL SURFACE FOR 33 DAYS. THE RATE IN THE REACTOR WAS SIMILAR TO THAT IN THE PREVIOUSLY-DESCRIBED BATCH REACTOR. N.EUROPAEA INCREASED IN THE GEL REGION FROM 0 TO 200 MU M DEPTH,AND P.DENITRIFICANS INCREASED FROM 0 TO 300 MU M DEPTH. FROM THE DISTRIBUTION OF N.EUROPAEN AND P.DENITRIFICANS,THE INNER SPACE OF THE TUBULAR GEL CAN BE CLASSIFIED INTO THREE REGIONS. THE FIRST REGION FROM 0 TO 100 MU M DEPTH IS THE AEROBIC REGION FOR AMMONIA OXIDATION. THE SECOND REGION FROM 100 TO200 MU M DEPTH CONSISTS OF THE AEROBIC REGION FOR AMMONIA OXIDATION AND ANAEROBIC REGION FOR DENITRIFICATION. THE THIRD REGION FROM 200 TO 300 MU M DEPTH IS THE ANAEROBIC REGION FOR DENITRIFICATION. THE USEFUL REGION FOR AMMONIAREMOVAL IS FROM 0 TO 300 MU M DEPTH. CONSEQUENTLY,AMMONIA CAN BE EFFECTIVELY REMOVED FROM WASTEWATER BY A SINGLE-PASS REACTOR CONTAINING THE TUBULAR GEL. A BIOREACTOR USING THIN TUBULAR GELS WOULD BE EFFECTIVE FOR DEVELOPING A NITROGEN-REMOVAL SYSTEM,BECAUSE THE SURFACE AREA PER VOLUME OF THE THIN TUBE IS LARGER THAN THA

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