報告書番号

U94011

タイトル（和文）

ワクチンによる魚病予防技術の開発１．ヒラメにおけるエリザ法の適用とワクチン投与の有効性

タイトル（英文）

DEVELOPMENT OF TECHNOLOGIES TO PREVENT FISH DISEASES BY VACCINATION 1. ADAPTATION OF ELISA TO DETECT SERUM ANTIBODIES AND EFFECT OF VACCINATION ON JAPANESE FLOUNDER

概要 （図表や脚注は「報告書全文」に掲載しております）

エドワジエラ症に対するワクチンを投与したヒラメの免疫反応を明らかにするために，ワクチン効果の指標の一つとされる抗体価の測定へのエリザ法の適用および，ワクチン投与後の防御免疫ならびに抗体価の測定を行い，以下の結果を得た。１．エリザ法（酵素抗体法）を新たにヒラメの抗体価測定に適用した。その結果，ヒラメの抗体価を精度よく測定することが可能となった。２．ヒラメに注射および浸漬によってワクチン投与したところ，浸漬法ではワクチン効果が見られなかったが，注射法による投与後に防御免疫の増強が認められた。防御免疫の増強は，投与７日後から３０日後までの間に，また，魚体重１００ｇあたり１０－１ｍｇ以上投与した時に認められた。また，平均体重３，１７，１４６，５１６ｇのヒラメについては，注射法では全てのサイズ，浸漬法では平均体重５１６ｇで血液中の抗体価の上昇が認められた。

概要 （英文）

IMMUNIZATION AND CHALLENGE EXPERIMENTS WERE CONDUCTED TO COMPARE THE EFFECT OF INJECTION AND IMMERSION METHODS IN VACCINATION AGAINST EDWARDSIELLOSIS OF JAPANESE FLOUNDER. VACCINE WAS MADE FROM FORMALIN-KILLED EDWARDSIELLA TARDA. AN ELISA (ENZYME-LINKED IMMUNOSORBENT ASSAY) WAS USED TO DETECT FLOUNDER SERUM ANTIBODIES,WHICH ARE BELIEVED TO BE RELATED TO THE EFFECT OF VACCINATION. (1)TO ADAPT AN ELISA,FLOUNDER SERUM ANTIBODY (LGM) WAS PURIFIED AND RABBIT ANTI-FLOUNDER LGM SERUM WAS PREPARED. THE ELISA HAD HIGH SENSITIVITY,COMPARED WITH THE AGGULUTINATION METHOD. (2)AMONG FISH VACCINATED BY INTRAPERITONEAL INFECTION AND IMMERSION,PROTECTIVE IMMUNITY WAS RECOGNIZED IN FISH VACCINATED BY INJECTION. HOWEVER,NO PROTECTIVE IMMUNITY WAS RECOGNIZED IN FISH VACCINATED BY IMMERSION. AFTER VACCINATION BY INJECTION,PROTECTIVE IMMUNITY WAS DETECTED FROM 7 TO 30 DAYS LATER IN FISH INFECTED WITH MORE THAN 10-1 MG/100 G B.W. OF VACCINE. AMONG FISH WEIGHING ABOUT 3,17,406 AND 516 G,SERUM ANTIBODIES WERE RECOGNIZED IN ALL FISH VACCINATED BY INJECTION AND IN FISH WEIGHING ABOUT 516 G VACCINATED BY IMMERSION. BASED ON THESE RESULTS,VACCINATION BY INTRAPERITONEAL INJECTION HAS SOME EFFECT IN PREVENTING EDWARDSIELLOSIS. IT IS SUGGESTED THAT FISH WEIGHINGABOUT 516 G ABSORBED VACCINE BY IMMERSION. FURTHER STUDIES ON THE IMMERSION METHOD ARE REQUIRED FOR MASS VACCINATION IN AQUACULTURE.

報告者

キーワード