報告書番号

U91064

タイトル（和文）

組織培養によるイチゴなどの種苗生産技術の開発（５）－イチゴカルスの固形培地上での再分化と発育に及ぼす培養環境－

タイトル（英文）

DEVELOPMENT OF TECHNOLOGY FOR PLANTLET PROPAGATION BY TISSUE CULTURE (5) -DIFFERENTIATION OF STRAWBERRY CALLUS AND DEVELOPMENT ON SOLID CULTURE MEDIA UNDER SEVERAL ENVIRONMENTAL CONDITIONS-

概要 （図表や脚注は「報告書全文」に掲載しております）

液体培養による培養苗の大量生産技術を開発するため，液体培養カルスの固形培地上での再分化と茎葉などの発育を促す培養環境条件を明らかにした。培養器の通気性および培地のゲルライト（ゲル化剤）濃度と液体培養カルスの再分化の関係を調べた。ゲルライト濃度が１．０と２．０％の培地では乾燥して枯死し，通気性の小さな培養器でゲルライト０．５％の培地において高い再分化率が得られた。移植操作の容易な固形培地として，ポリエステル繊維は，新鮮重の増加や芽と根の形成に優れ，発■ウレタンでは，根が伸長し固形培地に貫通した。固形培地上での培養では葉や根の発育が盛んで抜根の容易なポリエステル繊維粒が適当である。環境を制御できる大型の培養器を開発し，カルスを培養したところ，従来の培養器に比べて葉や根の形成率が高く、新鮮重と乾物重の増加が認められた。

概要 （英文）

LIQUID CULTURE IS ONE OF THE MOST IMPORTANT TECHNIQUES FOR DEVELOPMENT OF LARGE-SCALE PLANTLET PROPAGATION. IN THISRESEARCH,CALLUSES GROWN IN LIQUID MEDIA WERE CULTURED ON GELRITE SOLIDMEDIA OR OTHER SUPPORTING MATERIALS. THE ENVIRONMENTAL CONDITIONS WEREINVESTIGATED TO IMPROVE THE CALLUS DIFFERENTIATION RATIOS AND DEVELOPMENT OF SHOOTS AND ROOTS. AN EXPERIMENT WAS PERFORMED TO CLARIFY THE EFFECTS OF GAS EXCHANGE IN THE CULTURE VESSELS AND OF GELRITE CONCENTRATIONS ON FORMATION OF ADVENTITIOUS SHOOTS. CALLUS GROWTH WAS SUPPRESSED ON1.0% AND 2.0% GELRITE MEDIA. ADVENTITIOUS SHOOTS WERE FORMED BEST ON 0.5% GELRITE MEDIA IN TWO CLOSELY-CAPPED VESSELS. POLYESTER FIBER SOLID MEDIA PERMITTED EASY TRANSPLANTING OF CALLUSES AND ACCELERATED THE INCREASE IN FRESH WEIGHT OF THE TISSUES,AS WELL AS THE SHOOT AND ROOT FORMATION AND DEVELOPMENT. PARTICLE POLYESTER FIBER WOULD BE A SUITABLE SOLID-MADIA CULTURE METHOD BECAUSE OF THE ACTIVE SHOOT AND ROOT FORMATION AND THE EASE OF REMOVING ROOTS FROM THE MEDIUM. A LARGE-SCALE CULTURE VESSEL WITH CONTROLLABLE ENVIRONMENTAL CONDITIONS WAS DEVELOPED TO IMPROVE CALLUS GROWTH AND SHOOT AND ROOT FORMATION. THE FRESH WEIGHT AND DRY WEIGHT OF TISSUES INCREASED IN THIS VESSEL AND THE SHOOT AND ROOT FORMATION WAS ACCELERATED IN COMPARISON WITH THE GROWTH IN CURRENT VESSELS.

報告者

キーワード