報告書番号

U89035

タイトル（和文）

高能率魚類生産のための水質浄化技術の開発３．脱窒型から見た有用海洋性脱窒菌株の選抜

タイトル（英文）

DEVELOPMENT OF WATER PURIFICATION TECHNOLOGIES FOR INTENSIVE FISH CULTURE 3.SELECTION OF MARINE DENITRIFYING BACTERIA BY DENITRIFICATION PATTERN.

概要 （図表や脚注は「報告書全文」に掲載しております）

魚類飼育海水中に蓄積する硝酸の除去に有効な脱窒技術を確立するため，海洋性脱窒細菌の分離を行い，その脱窒型から有用菌株を選抜した。脱窒細菌の分離には集積培養が必要であり，また，集積培養法でも，硝酸集積培養法より亜硝酸集積培養法が，高い確率で脱窒細菌が分離できた。分離脱窒菌株の脱窒型を判別した結果，高能率魚類生産システムに有用であると考えられる脱窒型の菌株１６菌株を得た。これらの菌株は，硝酸の脱窒過程で亜硝酸を菌体外へ放出せず，最終産物としてガス状窒素を気相へ放出する。分離脱窒菌株は，その生理的性質から，９菌株がＰｓｅｕｄｏｍｏｎａｓ属と，７菌株がＡｌｃａｌｉｇｅｎｅｓ属と分類された。アセチレン阻害法を用いて，１６菌株の脱窒速度を亜酸化窒素の生成量として測定した結果，各菌株の脱窒速度は蛋白質１ｍｇ当り１３１～４３３０ｎｍｏｌＮ２Ｏ／ｈｒであり，菌株によって脱窒能力が異なること

概要 （英文）

USEFUL STRAINS OF MARINE DENITRIFYING BACTERIA WERE SELECTED BY THEIR DENITRIFICATION PATTERN TO ESTABLISH EFFECTIVE DENITRIFICATION OF NITRATE ACCUMULATING IN RECIRCULATING SEA-WATER AQUACULTURE SYSTEMS. DENITRIFYING BACTERIA FROM COASTAL SEDIMENTS OF ATSUMI BAY YOKOSUKA-ODAWA BAY,SENDAI BAY,SENDAI-GAMOU TIDELAND WERE SCREENED. ALSO,FILTER SAND FROM THE RECIRCULATING SEAWATER AQUACULTURE SYSTEM AT THE ABIKO RESEARCH LABORATORY,CRIEPI WAS SCREENED. DENITRIFYING BACTERIA WERE ISOLATED BY THE NITRATE OR NITRITE ADDED-ENRICHMENT CULTURE METHOD. EIGHTY-ONE OF 235 STRAINS ISLOLATED BY THE NITRATE METHOD WERE DENITRIFERS,AND 121 OF 145 STRAINS ISOLATED BY THE NITRITE METHOD WERE DENITRIFERS. SIXTEEN STRAINS THAT DID NOT PRODUCE NITRITE WERE SELECTED BY ESTIMATION OF THE DENITRIFICATION PATTERN. NINE OF THE 16 STRAINS WERE PSEUDOMONAS AND THE REST WERE ALCALIGENES. THE DENITRIFYING RATES OFTHE 16 STRAINS WERE MEASURED USING THE ACETYLENE INHIBITION TECHNIQUE UNDER ANAEROBIC CONDITIONS.THE DENITRIFYING ACTIVITIES RANGED FROM 131 TO 4330 NMOL/HR/MG PROTEIN.

報告者

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