報告書番号

U03027

タイトル（和文）

ＲＡＰＤ法を用いた植林地と自然林におけるマングローブの遺伝的多様性の比較

タイトル（英文）

A RAPD comparison of genetic divercity between plantation grown and natural grown mangrove

概要 （図表や脚注は「報告書全文」に掲載しております）

関西電力（株）がタイで進めているマングローブ植林における主要樹種（フタバナヒルギ、オオバヒルギ）について、植林地と周辺自然林の遺伝的多様性がどの程度異なるのかを明らかにするために、ＤＮＡから最も簡便に得られる多型（ＲＡＰＤ）を指標として解析を行った。解析には、バックグラウンドや泳動マーカー、特定バンドなどを内部指標として標準化を行った後に多型度、近縁度を算出し、クラスタリング等の統計手法を応用した。その結果、異個体群に属する同種マングローブ間の遺伝的多様性の比較にＲＡＰＤ法が有効であること、モデルとした植林個体群と自然林個体群の多様性はほぼ同程度であることが明らかとなった。

概要 （英文）

Plantation is one of the most effective ways to solve the problem of global warming and climate change. However, plantation usually uses seeds and/or plantlets from very few numbers of specified mother plants. This may gradually reduce and/or change a genetic diversity of the population, which is usually ensured in the surrounding natural forest at a level. Thus, it is important to investigate how the genetic diversity is kept in our plantation compared to the surrounding natural forest, and if those are far apart from each other, it is necessary to change the method of the plantation. On the other hand, a method using molecular biological technique is useful to distinguish the differences among plants belonging to the same species. Then, we tried to apply the technique based on the RAPD to show and compare the genetic diversity of a mangrove plantation and the surrounding natural forest situated in Thailand. Result indicated that there is basically no significant difference in the genetic diversity between our mangrove plantation and the surrounding natural forest. Also, it is possible to differentiate these two populations by the ratio of the component consisting of the polymorphism (RCP), even though the diversity level is the same between these two populations. This may be explained by small differences in each genetic background generated with the sampling time and situations.

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