報告書番号

U00077

タイトル（和文）

ラットの尿中神経伝達物質代謝産物の定量化手法の開発と回転円磁界の短期曝露影響

タイトル（英文）

The studies on biogenic amines and pteridines in urine of rats with an improved animal metabolism cage - the quantitative determination method and its application to the study of magnetic field exposure -.

概要 （図表や脚注は「報告書全文」に掲載しております）

実験動物に与える磁界曝露の影響を調べるため、一定時間毎に排尿を計測できる動物代謝ケージおよび最大0.5mTrmsの回転円磁界が発生可能な磁界曝露装置を製作した。本装置を用いて、ラットの尿を無侵襲かつ経時的に12時間毎、10日間に亘り計測し、生理活性アミン（エピネフリン、ノルエピネフリン）及びプテリジン化合物のビオプテリン変化を時系列的に調べた。この間、２日間の短期磁界曝露をストレスとして加えた｡その結果、エピネフリン変化には日周リズムが観察され、また一定のままであった｡ノルエピネフリンはケージに入れた直後は高く、交感神経系の活動が活性化されているものと推察される。ビオプテリン分泌には変化が見られなかった｡また、磁界の短期曝露による変化も観察されなかった。これらの結果はケージおよび磁界曝露が大きなストレッサーとして働いていないことを示している。

概要 （英文）

This report gives the experimental results dealing with the construction of animal metabolism cage to collect rat's urine and also the construction of magnetic field exposure system with the strength up to 0.5mTrms. Using this newly developed animal metabolism cage with magnetic field exposure system, the collection of rat's urine during 10 days with 12:12(LD) intervals was performed. The contents of epinephrine, norepinephrine and biopterin in rats'urine were analysed. During 10 days, the rat was exposed to circularly polarized magnetic field (0.5mT) with 2 days which is on the assumption that rat is under an acute stress. As a result, the volume in the rat's urine has regular day-night rhythm. The concentration of epinephrine has not changed, almost constant with regular rhythm. But just after keeping the rat inside an animal cage, the concentration of norepinephrine was increased and gradually declined along experimental day. This suggests that due to the change of environmental condition, stressor, the activation of the sympathetic nervous system appeared only just after staying rat inside animal metabolism cage. The biopterin has not changed during 10 days. An acute exposure to magnetic field has no influence on the change of biogenic amines and biopterin. Overall result is that our animal cage is useful to investigate the change of rat's metabolic product. From the estimation of the changes of biogenic amine and biopyterin, it is likely that the magnetic field may not be the stressor to animal.

報告者

キーワード