報告書番号

483009

タイトル（和文）

エネルギー源としてのバイオマスの探索と評価に関する研究第１報雌雄異株植物ナツメヤシのザイモグラムによる幼苗期雌雄判別法について

タイトル（英文）

STUDIES ON PLANT MATERIALS AS ENERGY RESOURCES (1) -SEX IDENTIFICATION BY AN ISOZYME METHOD IN DATE PALM(PHOENIX DACTYLIFERA L)-

概要 （図表や脚注は「報告書全文」に掲載しております）

雌雄異株植物であるナツメヤシの雌雄性をアイソザイム法によって幼苗期に検定できるか否かを雌樹，雄樹および実生苗の葉片を用いて検討した。エステラーゼザイモグラムには雌雄性による差異は認められなかったが，パーオキシダーゼザイモグラムでは雌雄性による差異が認められた。また，雌雄性未知の実生苗においても成樹と同様な二種類のパーオキシダーゼザイモグラムが得られた。この結果，アイゾザイム法により幼苗期に雌雄性を判別する可能性があると推定された。

概要 （英文）

ISOZYME METHOD HAS PROVED EFFECTIVE IN ADVANCING GENETICAL AND PHYSIOLOGICAL STUDIES. IN THIS STUDY A POSSIBILITY TO IDENTIFY SEX DIFFERENCE IN DIOECIOUS DATE PALM BY THE METHOD WAS PURSUED.PEROXYDASE AND ESTERASE ZYMOGRAMS WERE EXAMINED BY STARCH GEL ELECTROPHORESIS METHOD IN LEAF EXTRACTS FROM MALE AND FEMALE ADULT PLANTS,AND FROM YOUNG SEEDLINGS OF 3RD TO 5TH LEAF STAGE. NO DIFFERENCE WAS OBSERVED IN THE ESTERASE ZYMOGRAMS,WHILE CLEAR DIFFERENCES WERE FOUND IN PEROXYDASE ZYMOGRAMS BETWEEN THE MALE AND THE FEMALE MATERIALS.THAT IS,INTHE FEMALE MATERIALS TWO BANDS WERE SHOWN AT THE CATHODAL PORTION OF THE ZYMOGRAMS,BUT WERE NOT IN THE MALE MATERIALS.SIMILAR DIFFERENCES WERE GIVEN IN THE YOUNG SEEDLINGS,THUS THEY WERE DIVIDED INTO TWO TYPES.ONE TYPE RESPOND TO THE MALE ADULT AND ANOTHER TO THE FEMALE ONE IN THE ZYMOGRAM PATTERNS. ON CONCLUSION A POSSIBILITY IS GIVEN TO IDENTIFY SEX DIFFERENCE BY THE ISOZYME METHOD EVEN AT 3RD TO 5TH LEAF STAGE OF YOUNGSEEDLINGS IN DATE PALM PLANT.

報告者

キーワード